营养物失衡是维持癌细胞恶性生长的因素之一──DMEM培养基使黑色素瘤B16细胞的恶性生长抑制

Unbalancing Nutrition is One of the Factors Maintained the Malignant Growth of Cancer Cells DMEM Medium Inhibited the Malignant Growth of Mouse Melanoma B16 Cells

对小鼠黑色素瘤B1 6细胞在DMEM和RPMI1 640两种培养基中培养产生的活细胞数和黑色素量进行了检测 .结果显示 ,该癌细胞在DMEM培养基中培养产生的活细胞数比在RPMI1 640培养基中培养产生的活细胞数平均减少 87.9% ,而黑色素量平均增加795 % .以“黑色素量 /活细胞数”计算分化指数 ,再以“DMEM中的分化指数 / 1 640中的分化指数”计算DMEM相对于 1 640的对B1 6细胞的分化指数 ,显示DMEM中的分化指数平均是 1 640中的分化指数的 67.8倍 .

The amounts of living cells and melanin of mouse melanoma B16 cultured in DMEM and RPMI1640 media are tested. The results show that the amount of living cells of mouse melanoma B16 cultured in former medium is averagely lowered by 87.9% and the amount of melanin of mouse melanoma B16 cultured in later medium is averagely increaseded by 795%. The differentiation indexes of mouse melanoma B16 cells cultured in DMEM and RPMI1640 media are calculated as 'melanin amount/living cells amount', and the relative differentiation index of mouse melanoma B16 cells cultured in DMEM medium is calculated as 'differentiation index in DMEM/differentiation index in RPMI1640'. It shows that the differentiation index of mouse melanoma B16 cells cultured in DMEM is averagely 67.8 times of that in RPMI1640 medium. According to these results, it is proposed that unbalancing nutrition is one of the factors that maintain the malignant growth of cancer cells.