肝再生刺激因子对小鼠实验性急性肝损伤的保护作用

PROTECTIVE EFFECT OF HEPATIC STIMULATOR SUBSTANCE AGAINST EXPERIMENTAL ACUTE LIVER FAILURE IN MICE

本工作采用雄性初断乳 SD 大鼠肝按 LaBrecque 法提取肝再生刺激因子(HSS),用四氯化碳和半乳糖胺分别损伤小鼠肝来研究 HSS 的保肝作用。结果如后:(1)HSS 可使 CCl_4致肝损伤小鼠的血清 GPT 和 GOT 升高幅度降低,并呈量效关系。(2)肝组织切片表明 HSS 可使 CCl_4损伤肝组织的程度减轻。(3)肝组织化学法表明 HSS 可使 CCl_4损害肝细胞线粒体琥珀酸脱氢酶的活性恢复。(4)胰岛素-胰高糖素可降低半乳糖胺所致的小鼠死亡率,减弱对肝组织的损害和刺激肝细胞增殖,这项实验可作为 HSS 具有保肝作用的证据。

A hepatic stimulator substance(HSS)was extracted from the liver of maleweanling SD rats according to the method of LaBrecque.The mice were in-jected with carbon tetrachloride or D-galactosamine to induce hepatic injuriesand the protective effect of HSS on thus induced hepatic damage was investi-gated.The results were as follows:(1)HSS could suppresses the elevation of sGPTand sGOT induced by carbon tetrachloride intoxication in a dose-dependentmanner.(2)Hepatic histological findings indicated that the degree of CCl_4 orD-galactosamine-induced hepatic lesions could be lessened by HSS.(3)CCl_4-induced reduction of hepatic mitochondrial succinic dehydrogenase activitycould be restored by HSS.(4)Insulin-glucagon enhanced the survival of D-galactosamine intoxicated mice and stimulated hepatocyte proliferation,thusshowing less pronounced hepatic damage.