肝细胞癌细胞凋亡与浸润转移潜能的关系

Relationship between apoptosis and invasive and metastatic potential of hepatocellular carcinoma

目的 研究肝细胞癌细胞凋亡和浸润转移潜能的关系。方法 肝细胞癌 5 7例 ,用流式细胞仪检测细胞凋亡率 (AR)和细胞增殖活性 [用S期细胞比率 (SPF)和增殖指数 (PI)表示 ] ,用链霉亲合素 生物素酶复合物免疫组织化学检测 p2 1、p170和ndpk。 结果 AR (1.77± 0 .19) % ,SPF(12 .5 5± 0 .68) % ,PI(2 0 .91± 1.12 ) % (r =-0 .173 ) ;AR≤ 1.77%者 p170 (+ )、p2 1(+ )、ndpk(+ )分别为 61.3 6%、68.18%、5 2 .2 7% ,AR >1.77%者p170 (+ )、p2 1(+ )、ndpk (+ )分别为2 3 .0 8%、3 8.46%、84.62 % (P均 <0 .0 5 ) ;浸润转移组ndpk(+ ) 43 .75 % ,p2 1(+ ) 75 .0 0 % ,p170(+ ) 65 .63 % ,AR(1.12± 0 .16) % ,PI(2 3 .78± 1.48) % ,SPF(13 .90± 0 .99) % ,非浸润转移组ndpk(+ ) 80 .0 0 % ,p2 1(+ ) 44 .0 0 % ,p170 (+ ) 3 6.0 0 % ,AR (2 .3 2± 0 .5 2 ) % ,PI(18.5 3± 0 .82 ) % ,SPF(11.43± 0 .70 ) % (P均 <0 .0 5 )。结论 肝细胞癌细胞凋亡与其浸润转移潜能呈负相关 ,且与细胞增殖活性、ndpk、p2 1和 p170等多因素有关。

Objective To study the relationship between apoptosis and invasive and metastatic potential of hepatocellular carcinoma (HCC).Methods Apoptotic rate (AR),proliferative index (PI) and S phase fraction (SPF) were measured by flow cytometry;p170,p21 and ndpk by strept avidin biotin complex immunohistochemical technique in 57 cases of HCC.Results AR (1.77±0.19)%,SPF (12.55± 0.68)%,PI (20.91±1.12)% (r=-0.173).p170,p21 and ndpk positive rate was 61.36%, 68.18%,52.27%,respectively in the group with AR≤1.77%,while 23.08%,38.46%, 84.62%, respectively,in the group with AR>1.77% (all P<0.05).In the group with positive invasiveness and metastasis,ndpk(+) was 43.75%,p21(+) 75.00%,p170(+) 65.63%,AR (1.12± 0.16)%, PI (23.78±1.48)%,SPF (13.90±0.99)% respectively;In the group with negative invasiveness and metastasis,ndpk(+) was 80.00%,p21(+) 44.00%,p170(+) 36.00%,AR (2.32± 0.52)%, PI (18.53±0.82)%,SPF (11.43±0.70)% respectively.Conclusion Apoptosis of HCC might have negative correlation with its invasive and metastatic potentials,and have relations to multiple factor such as proliferative activity,p21,p170 and ndpk etc.