摘要
对以硅胶为吸附剂的 1 .5cm× 40cm色谱柱分离卵磷脂的操作条件进行了研究 .利用薄层层析法确定了洗脱剂的组成为甲醇 /氯仿混合液 (pH值 3～ 4) .通过洗脱方式及梯度差实验 ,得出梯度差为V(CH3OH)∶V(CHCl3) =(1∶2 )～ (2∶1 )的凹形梯度洗脱为最佳洗脱方式的结论 ,其洗脱剂用量仅为柱体积的5～ 6倍 ,洗脱时间为 6h左右 .
The conditions which prepare high-purity Lecithin with 1.5 cm×40 cm silica gel chromatographic column are systematically studied. The component of eluent is CH 3OH-CHCl 3 solvents with pH 3～4. The optimum gradient elution process is concave gradient eluting with the gradient of V(CH 3OH)∶V(CHCl 3)=(1∶2)～(2∶1). The volume of eluent is only 5～6 times of column volume, and the time of eluting is about 6 h.
基金
湖北省教育厅自然科学基金资助项目 (98B0 0 6 9)
