纤维素三苯甲酯手性固定相的制备及对映体的拆分

Preparation of Cellulose Tribenzoate as Chiral Stationary Phase in HPLC and Separation of Enantiomers on It

用微晶纤维素和苯甲酰氯的超声反应合成了纤维素三苯甲酯 ( CTB) ,并将其涂敷于硅胶上 ,用作液相色谱手性固定相。在正相条件下 ,用此手性固定相对一系列外消旋缩水甘油芳香基醚类化合物进行了拆分 ,并对拆分机理进行了探讨。

Cellulose tribenzoate chiral stationary phase(CTB CSP) was prepared by a modified procedure, in which CTB was synthesized by the ultrasonic reaction of benzoyl chloride with microcrystalline cellulose and then was coated on the silica gel. Enantiomers of a series of glycidyl aromatic ethers were separated by CTB CSP in normal chromatographic conditions. The chiral recognition mechanism was discussed for the separation of these chiral glycidyl aromatic ethers. The effects of different alcohols in mobile phase on the chiral separation of glycidyl m tolyl ether enantiomers were also investigated.