直肠粘液腺癌bax及bcl-2基因的表达及意义

Expression of bax and bcl-2 gene in mucous adenocarcinoma of the rectum

目的观察直肠粘液腺癌组织中bax和bcl 2基因的表达并探讨其临床意义。方法应用免疫组织化学ABC法 ,以bax及bcl 2单克隆抗体标记 38例直肠粘液腺癌组织 ,并分析与临床病理学指标及预后的关系。结果bax的阳性表达率在DukesA和B期为 77 8% ,在DukesC期为45 0 % ,二者相比差异有显著意义 (χ2 =4 2 6 0 ,P =0 0 39) ;bcl 2的表达与各病理学指标无明显关系 ,但bax与bcl 2阳性表达强度之比值与Dukes分期 (P =0 0 4)和病人 5年生存率 (P =0 0 0 1)相关。结论bax及bcl 2在直肠粘液腺癌中普遍表达。

ObjectiveTo determine the relationship between the expression of bax and bcl-2 gene in mucous adenocarcinoma of rectum and patient′s prognosis.MethodsImmunohistochemical method was used to evaluate monoclone antibody of bax and bcl-2 protein in 38 cases of mucous adenocarcinoma of the rectum. ResultsThe positive-staining rate of bax was 77.8% in Dukes′ A and B stage compared to 45.0% in Dukes′ C stage( χ 2=4.260,P=0.039).The intensity of positive-staining of bax over bcl-2 was significantly correlated with tumor stage(P=0.04) and the survival (P=0.001).ConclusionThe expression rate of bax and bcl-2 gene was very high in mucous adenocarcinoma of large bowel, and it might have a prognosis implications in patients with colonic mucous adenocarcinoma.