摘要
目的 探讨人肝硬化肝组织和肝硬化肝细胞生长激素受体的表达变化。方法 采用放射配体法、逆转录聚合酶链反应 (RT PCR)检测 32例癌旁肝硬化肝组织和 6例癌旁肝硬化肝细胞中生长激素受体及其mRNA的表达水平 ,并分别与 5例正常肝组织、3例正常肝细胞比较。结果 人肝硬化肝组织表达生长激素受体 ,其表达数量较正常肝组织明显减少 [RT,(2 2 4± 5 0 )fmol/mg蛋白 ,n =32vs (41 3± 5 3)fmol/mg蛋白 ,n =5 ;P <0 0 5 ],并且随着肝硬化程度的加重进一步减少 (P <0 0 5 ) ;人肝硬化肝细胞生长激素受体的位点数量较正常肝细胞明显减少 [位点 ,(2 4 0± 0 93)× 10 4/细胞 ,n =6vs (4 6 8± 1 14 )× 10 4/cell,n =3;P <0 0 5 ],人肝硬化肝组织生长激素受体mRNA的表达量也较正常肝组织明显减少 [riOD ,30 8%± 8 2 % ,n =32vs 4 4 9%± 6 3% ,n =5 ;P <0 0 5 ],并且随着肝硬化程度的加重进一步减少 (P <0 0 5 )。结论 肝硬化肝细胞表达生长激素受体 ,其表达水平降低 ;肝硬化越严重 ,该表达下调越明显。
Objective To investigate the expression of growth hormone receptor in human cirrhotic livers. Methods Radio ligand binding assay and reverse transcription polymerase chain reaction (RT PCR) were used to examine the expression of growth hormone receptor and its mRNA in cirrhotic liver tissues near cancer in 32 cases and cirrhotic liver cells near cancer of 6 patients undergoing radical resection of liver cancers. The results were compared with those in 5 specimens of normal liver tissue and 3 specimens of normal liver cells. Results The binding capacity of growth hormone receptors in human cirrhotic liver tissues was significant lower than that in normal control [RT, (22.4±5.0) fmol/mg protein, n =32 versus (41.3±5.3) fmol/mg protein, n =5; P <0.05] and decreased further along with the aggravation of cirrhosis ( P <0.05). The number of binding sites of growth hormone receptors in human cirrhotic liver cells were significantly lower than that in normal control [Site, (2.40±0.93)×10 4/cell, n =6 versus (4.68±1.14)×10 4/cell, n =3; P <0.05]. The expression of growth hormone receptor mRNA in human cirrhotic liver tissues was significant lower than that in normal control [riOD,30.8%±8.2%, n =32 versus 44.93%±6.25%, n =5; P <0.05] and decreased further along with the aggragation of cirrhosis ( P <0.05). Conclusion The expression of growth hormone receptor in human cirrhotic liver cells is downregulated. The more severe liver cirrhosis the more the downregulation. The decreased expression of growth hormone receptor mRNA may account for the downregulated expression of growth hormone receptor in cirrhotic liver tissues.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2002年第3期168-171,共4页
National Medical Journal of China
基金
广东省自然科学基金资助项目 (9842 1 3 )
瑞士Serono中国公司专项基金资助 (98 6 6 )