维生素E对肝脏星状细胞Ⅰ型胶原、TGFβ_1和TIMP_2表达的影响

Effects of vitamin E on the expression of type Ⅰ collagen, TGFβ_1 and TIMP_2 of hepatic stellate cells

目的 探讨维生素E对肝脏星状细胞 (HSC)Ⅰ型胶原、转化生长因子 β1(TGFβ1)和基质金属蛋白酶组织抑制剂 2 (TIMP2 )表达的影响。方法 体外培养的HSC ,经 1mg/L维生素E作用后 ,用免疫组织化学法检测Ⅰ型胶原表达 ;原位杂交技术检测TGFβ1和TIMP2 mRNA表达。结果 实验组中维生素E能使HSCⅠ型胶原表达明显少于对照组 (P <0 .0 1) ,但对TGFβ1及TIMP2 mRNA表达无影响。结论 维生素E抗肝纤维化作用可以通过抑制Ⅰ型胶原表达实现。

Objective To investigate the influence of vitamin E on type Ⅰ collagen, messenger RNA expression of transforming growth factor β 1 (TGFβ 1) and tissue inhibitor of matrix metalloproteinases-2 (TIMP 2) in hepatic stellate cells (HSC). Methods HSC s were cultured in vitro, the effect of vitamin E on type Ⅰ collagen was observed by mean of immunohistochemistry; mRNA expression of TGFβ 1 and TIMP 2 were investigated with in situ hybridization. Results In experiment group, vitamin E markedly inhibited expression of type Ⅰ collagen of HSC as compared with control group, but could not suppress mRNA expression of TGFβ 1 and TIMP 2.Conclusions Anti-fibrotic effect of Vitamin E may be implemented through inhibiting the expression of type Ⅰ collagen.