谷氨酰胺对大鼠放射性肠粘膜损伤的保护作用

The Protection of Glutamine on Radiation-induced Intestinal Mucosa Injury in Rats.

目的 观察谷氨酰胺对放射性肠粘膜损伤的保护作用。方法 将 3 0只大鼠随机分为正常对照组 (A组 )、照射对照组 (B组 )和谷氨酰胺保护组 (C组 )。大鼠全腹照射 10 0 0cGy。C组自照射前 1d开始灌服谷氨酰胺。 4d后处死大鼠 ,检查肠道细菌移位情况、血中内毒素水平及小肠粘膜病理形态学改变。结果 A组无细菌移位 ,B组细菌移位最为明显 ,C组细菌移位远不及B组明显 ;A组内毒素含量极低 ,B组内毒素含量明显升高 ,C组内毒素含量明显低于B组 ;A组肠粘膜正常 ,B组肠粘膜绒毛水肿 ,炎性细胞浸润 ,部分上皮细胞脱落 ,C组绒毛轻度水肿 ,未见明显细胞脱落。结论 全腹照射能明显损伤小肠粘膜 ,引起细菌移位和内毒素血症 ,谷氨酰胺能明显减轻射线对小肠粘膜的损伤。

Objective To observe the protection of glutamine on radiation-induced intestinal mucosa injury.Methods Thirty rats were randomly divided into normal control group(group A),radiation control group(group B)and glutamine protection group(group C).The rats were received abdominal radiation of 1 000 cGy.Feeding glutamine began since the day before radiation in group C.Four days later,the rats were killed,and the intestinal bacteria translocation,the concentration of endotoxin in blood and pathological changes of intestinal mucosa were measured or observed.Results Bacteria translocation was not found in group A,but evident in group B,and much lighter in group C than in group B.The concentration of endotoxin in blood was very low in group A,very high in group B.but much lower in group C than in group B.The villus edema,mucosa infiltrated with informative cells and epithelial exuviation were found in group B,but these pathological changes were much lighter in group C,and not found in group A.Conclusion Whole abdomen radiation will evidently cause intestinal mucosa injury in rats,and bacteria translocation and endotoxinemia would occur.Glutamine can prevent those changes.