HCMV抗原、抗体和DNA的同时检测在慢性肾盂肾炎中的意义

Significance of Simultaneous Detection of Cytomegalovirus Antigen Antibody and DNA in Chronic Pyelonephritis

目的 进一步探讨人巨细胞病毒 (HCMV)感染与慢性肾盂肾炎的关系及同时检测 HCMV抗原、抗体和 DNA三项指标的意义。方法 对 95例慢性肾盂肾炎患者和 83例正常对照 ,分别用免疫斑点法测尿中 HCMV抗原 ,酶联免疫吸附测定 (ELISA)测血清中 HCMV抗体 (Ig G型 ) ,聚合酶链反应 (PCR)测尿中 HCMV- DNA。结果 患者组 HCMV抗原、抗体 (Ig G型 )及 DNA阳性率分别为 5 5 .8% ,82 .1 % ,2 4 .2 % ,抗体 (Ig G型 ) S/N值 (标本 45 0 nm吸光度 /阴性对照 45 0 nm平均吸光度 )为 3 .40± 0 .86 ,均明显高于正常对照组。三项指标的出现并非完全一致。结论 HCMV感染可能与慢性肾盂肾炎有关 。

Aim To further research into the relationship between chronic pyelonephritis and human cytomegalovirus(HCMV)infection and the significance of simultaneous detection of HCMV antigen,antibody and DNA.Methods In 95 patients with chronic pyelonephritis and 83 normal control persons,HCMV antigen in urine was detected by dot immunobinding assay,HCMV antibody(IgG)in serum by enzyme linked immunosorbent assay(ELISA),and HCMV DNA in urine by polymerase chain reaction(PCR).Results In patient group,the positive rates of HCMV antigen,antibody(IgG) and DNA were 55 8%,82 1% ,and 24 2% respectively,and the S/N tatio (sample absorbance in 450 nm/negative control average absorbance in 450 nm)was 3 40±0 86,which all were much higher than those in normal control group.HCMV antigen,antibody and DNA were not all unanimous.Conclusion Chronic pyelonephritis may be related to HCMV infection and the simultaneous detection of HCMV antigen,antibody and DNA may have more significance.