农抗5102产生菌原生质体融合育种的研究——Ⅳ.融合子FR-008的验证及其产生新活性物质的分离和鉴别

Studies on Fusion Breeding of Protoplasts from Antibiotic-producing Strain 5102 Ⅳ. Verification of Fusant FR-008, Isolation and Characterization of Its New Antimicrobial Substance

本文在探索5102-1号抗生素液相色谱(HPLC)分析法的基础上,从出发菌株10-22和融合子FR-008的发酵物中均分离出5102-1号抗生素的主要组分,进一步证实融合于FR-008为吸水链霉菌应城变种种内融合的重组子。实验还从融合子FR-008的发酵物中分离和纯化了出发菌株不能产生的活性物质,通过与浓硫酸和浓盐酸反应呈深兰色,紫外可见光谱在403,380,360,340um处有吸收峰等特征,表明它是一种七烯大环内酯类抗生素。在建立该抗生素中芳香残基的液相色谱分析法时,证明其中含有对氨基苯乙酮残基。用氨基酸分析仪测定经硫酸甲醇醇解和醇解产物再水解后的抗生素样品,表明它含有与已知七烯大环内酯类抗生素不同的氨基糖残基,其详细结构有待进一步研究确定。

On. the basis of the scrutiny of the high performance liquid chroma-tography of Antibiotic 5102-1, the principal components of Antibiotic5102-1, which are active in the inhibition of Pellicularia sasakii and ableto produce typical abnormal branches, have been isolated from the star-ting strain 10-22 and the fermented product of fusant FR-008. The resultfurther verifies that FR-008, of which the aerial hyphae and culturalcharacteristics are not exactly the same as those of the starting strain, isa recombinant of intraspecific fusion of Streptomyces hygroscopicus var.yingchengensis. In the experiment, a new antimicrobial substance hadbeen isolated and purified from the fermented product of fusant FR-008.The substance show a color of dark blue when reacted with concentratedsulphuric acid and concentrated hydrochloric acid and display an absorp-tion peak at 403, 380, 360, 340nm respectively in the UV-VIS absorp-tions. These characteristics indicate that it is a kind of heptaene macro-lide antibiotic. In the process of liquid chromatography for aromatic mo-iety, this antibiotic is found to contain p-amino-acetophenone moiety.Sample of methanol hydrolysis and hydrolysis of methanol-hydrolisedsample of the antibiotic have been determined by means of amino acidanalyser, and are found to contain aminosugar moiety different fromthat in all the other heptaene macrolide antibiotics. The deatiled structureof the aminosugar moiety has yet to be identified in further studies.