猪基因组文库的构建及其生长激素基因的分离

Construction of Porcine Genomic Library and Isolation of Porcine Growth Hormone Gene

本工作以长白猪为材料,分别用Charon28及EMBL 3为载体,构建了猪的基因组文库。用牛生长激素基因为探针对基因文库进行筛选,由两个基因文库中各获得一个阳性克隆λPGH1,λPGH2。其后,以质粒pUC19为载体对λPGH1进行了亚克隆。通过对亚克隆pPGH的酶切图谱及其Southern杂交结果的分析表明,在pPGH中含有完整的猪生长激素基因。

Two porcine genomic libraries have been constructed using both pha-ge Charon 28 and EMBL3 as the vectors and two positive plaques(λPGH_1, λPGH_2) have been isolated using bovine growth hormone gene(bGH) as the hybridization probe. The gene within the λPGH_1 wassubcloned into plasmid pUC19. By digestion with 9 different restrictionenzymes and Southern hybridization, it showed that the plasmid pPGHcontains full length porcine growth hormone gene.