均匀设计在L-异亮氨酸发酵中的应用

Application of Uniform Design in L-isoleucine Fermentation

本试验用均匀设计方法考查了9种培养基组成对L-异亮氨酸产生菌钝齿棒状杆菌(Cory-nebacterium crenatum)A11发酵的影响,获得优化配比为:葡萄糖12%,硫酸铵2%,磷酸二氢钾0.1%,磷酸氢二钾0.45%,硫酸镁0.055%,生物素1μg,硫胺素15μg,硫酸亚铁2ppm,硫酸锰2ppm,碳酸钙4%,pH7.0～7.2。在此条件下,菌株A11可产生L-异亮氨酸15.1mg/ml。与正交设计相比,均匀设计具有试验次数少,工作效率高和便于分析因素影响等优点。

Uniform design was employed in our paper to test the influencesof nine medium substrates on the fermentation of L-isoleucine produ-cer Corynebacterium crenatum All. The optimum medium compositionswere as follows: glucose 12%, (NH_4),SO_4 2%, KH_2PO_4 0.1%,K_2HPO_40.45%, MgSO_4· 7H_2O 0.055%, biotin 1μg, thiamine 20μg, FeSO_4. 7H_2O2ppm,MnSO_4·4H_2O 2ppm,CaCO_3 4%,pH7.0--7.2. Under these conditionsthe amount of L-isoleucine produced by All was up to 15. 1mg/ml. Uniform design, compared with orthogonal design, has advantagesof less times of test, higher work efficiency and easier description offactor influences.