有机溶剂微扰葡萄糖淀粉酶时的紫外、荧光和红外光谱

THE UV, FTIR AND FLUORESCENCE SPECTRA OF GLUCOAMYLASE WITH SOLVENT-PERTURBATION

本文结果表明:(1)酶体系中随有机溶剂含量增加,紫外吸收亦随之增加,表明芳香氨基酸更充分地暴露,分子更趋于伸展;(2)酶的荧光发射随有机溶剂量增加而稍有变化,但当溶剂量达到一定值时[对乙二醇为35％(V/V)],荧光发射强度显著增加,表明酶分子构象受溶剂影响有一个极限值;(3)酶的傅立叶红外光谱表明,有机溶剂侧链的疏水性愈强,微扰后C=O,C—N,O—H等共价键的吸收峰变得愈宽,愈强,肽链愈伸展。

The UV, FTIR and fluorescence spectra differences of glucoamylase in the presence and absence of organic solvent e. g. acetone, methanol, propanol, dioxane, ethylane glycol etc. show that the aromatic amino acid (Trp, Tyr, Phe) are exposed after solvent-perturbation and then the conformational change of glucoamylase may be occurred with fast inactivation of the enzyme.