硒抗辐射损伤作用的探讨

EFFECT OF SELENIUM ON RADIATION INJURY

小鼠分次腹腔注射1.5mg Se/kg后,用^(60)Co 6.0Gy一次照射,观察硒对血及肝脏亚细胞组分线粒体GSHPx活性,LPO含量改变和~3H-TdR掺入的影响,以及在不同时间血和肝的GSHPx活性变化。并对不同剂量硒对LPO形成的影响进行了研究。结果表明,给硒及^(60)Co照射后小鼠体内的GsHPx活性显著增高;硒表现一定的抗辐射损伤作用和对损伤机体的恢复作用。另一方面,当给硒量在0.5mg/kg以上时,随着硒浓度的增加,LPO也增加,存在剂量效应关系。

After ^(60)Co irradiation 6.0Gy and/or administration of selenium (1.5mg Se/Kg, Selen- ite), the activity of glutathion peroxidase (GSHPx), the rate of lipid peroxide (LPO) formation and the incorporation of ~3H-TdR in the subcellular fractions of mouse liver and blood were studied. The results showed that the blood and liver mitochondrial GSHPx rose in the 1—4th days, dropped to subnormal level in the 5—12th days and then recovered in the 13—18th days. Se treatment had a markedly protecting effect to mouse on this change. It was also found that the LPO contents of blood and liver subcellular fractions were elevated significantly by ^(60)Co irradiation and small doses of Se had an inhibiting action on such an elevation. These results were supported by the experiment of incorporation rate of ~3HTdR in liver cell. However attention should be paid to a dose-effect relationship between the protecting effect and the toxicity of Se. It was found that when the dose of Se was over 0.5mg/Kg, the more Se was given the more LPO was formed.