几种哺乳类动物胸主动脉中的蛋白聚糖

PROTEOGLYCANS IN AORTAS FROM SEVERAL SPECIES OF MAMMALS

本文报道了北京(As高发)、广西南宁(As低发)两地区的人、兔(“易感”As)及狗(“不易感”As)胸主动脉中三种蛋白聚糖(CSPG,DSCSPG及HSPG)的含量。结果表明:a.广西南宁样品的总PGs,HSPG及DSCSPG含量均高于北京,尤以HSPG差异显著。b.兔、狗胸主动脉PG总量均低于人的。其中HSPG,CSPG含量及百分率以及兔的DSCSPG含量亦低于人的相应PG含量,而狗的DSCSPG含量与人的类似。c.南宁人及狗的DSCSPG含量及相对百分率虽高,但其DSCSPG中DS链所占相对百分率低于北京人和兔的。以上结果提示动脉壁PG质与量的差异可能与AS发病有关。

For the study on the relationship between arterial proteoglycans (PG_s) and atherosclerosis (As), PG_s of aortic intima-media of man (from Beijing with higher and Nanning in Guang Xi province with lower prevalence of As), rabbit and dog were separated successively on DEAE-Sephacel ion exchange and gel filtration column chromatography. Three types of PG, namely heparan sulfate PG(HSPG), chondroitin sulfate PG (CSPG) and dermatan sulfate-chondroitin sulfate PG(DSCSPG) were characterized and quantitated. Glycosaminoglycans from DSCSPG were run on cellulose acetate membrane and the relative percentages of DS and CS were calculated densitometrically on stained strips.The results revealed: 1. The contents of total PG., HSPG and DSCSPG in Nanning sample were significantly higher than the corresponding ones in Beijing sample; 2. The contents of total PG_s in aortas of rabbit and dog were lower than that of man, among which the contents and relative percentages of CSPG, HSPG of rabbit and dog, the content of DSCSPG of rabbit were also lower than that of corresponding PG in man; while the DSCSPG content of dog were similar to Nanning man; 3. Lower relative percentages of DS in DSCSPG from Nanning man and dog in comparing with those from either Beijing man or rabbit evne though the content and relative percentage of DSCSPG of Nanning man and dog were higher. As HSPG and DSPG were known to be antiproliferative to arterial smooth muscl tells and of higher binding affinity to plasma low density lipoprotein respectively, the above results might be implicated in the lower prevalence of As in Nanning man and 'resistant' co As of dog.