麦类作物抗全蚀病室内苗期接种方法与评价指标

INOCULATION METHODS AND EVALUATION INDICATORS OF CEREAL RESISTANCE TO 'TAKE-ALL' DISEASE AT SEEDLING STAGE IN GREENHOUSE

利用生物统计学方法,研究了室内苗期评价麦类作物抗全蚀病鉴定的方法和指标,证明菌饼和玉米砂接种法均可用于麦类作物的抗病性评估,但以菌饼接种法接种较好。该法简便易行,接菌量均匀,有利于品种间的相互比较。对菌饼和玉米砂接种法接种后30d的发病指标进行了通径相关分析和主成分分析。在12项发病指标中,只有皮层褐化长一个指标入选主成分,表明皮层褐化长度可反映品种的抗病性差异,该指标可用于室内苗期抗全蚀病资源的大量筛选。

Inoculation methods and disease indicators of cereal resistance to take-all disease at seedling stage in greenhouse were assessed by biological statistics. The results showed that the inoc-ula of both fungal disc and maize meal could be used in take-all resistance evaluation. However, the former was much better than the latter one because of easy performance, well-distributed inoculum density in each treatment, which resulted in reliable comparison between varieties tested. Twelve disease indicators after 30 days of inoculation were assayed using path analysis and principal components analysis, and only one indicator (length of cortical browning) had been selected. This result indicated that the length of cortical browning could be used in mass screening of the cereal resource resistance to take-all disease at seedling stage in greenhouse.