摘要
从双胸蚓组织中分离出水解胶原的酶(Ⅰ、Ⅱ、Ⅲ)。其中酶Ⅱ是一不具亚基结构的大分子糖蛋白,酸性氨基酸含量高于碱性氨基酸,缺乏胱氨酸。最适pH6～7,最适温度35～45℃,80℃10分钟酶失活。Ca^(++)、Mg^(++)、Ba^(++)、Mn^(++)促进酶活性,Co^(++)、Hg^(++)、EDTA、巯基化合物及正常人血浆抑制酶活性。胶原酶解产物在PAGE图谱上表现为泳动较快的新带。
Collagenases (Ⅰ . Ⅱ . Ⅲ) were extracted from the tissue of the Lumbri-cidae Bimastos. The collagenase Ⅱ was proved to be glycoprotein with molecular weight of 92900 and appeared to be a monomer.The enzyme contained higher amount of acidic amino acids than basic ones, and no cystine. Its optimum pH was between 6 and 7, optimum temperature was between 35℃ and 45℃ . Lose of activity occured after standing for 10 min, at 80℃. Collagenolytic activity was enhanced by Ca+ + 、 Mg+ + 、 Ba+ + 、 Mn+ + 、 but inhibited Co++、 Hg+ + 、 EDTA、 SH-compounds and normal plasma. Enzymatic attack on the collagen molecule resulted in the appearance of some new, faster moving bands in disc electrophoretogram.
基金
国家基金委员会
关键词
蚯蚓
胶原酶
纯化
Earthworm
Collagenase
Purification
