磺脲受体1基因多态性与血脂的相关性研究

An investigation on the relationship between sulfonylurea receptor 1 gene intron 24-3t/c polymorphism and blood lipids in Chinese population

目的 研究中国北方人群磺脲受体 1(SUR1)基因 2 4内含子 - 3t/ c多态性与血脂的相关性。方法 研究对象为 132对 2型糖尿病的病例对照和 2 82个 2型糖尿病家系。用聚合酶链反应 (PCR) -长度多态性的方法检测磺脲受体 1基因 2 4内含子 - 3t/ c多态性。统计方法采用 t检验、多元线性回归和以家系为基础的相关性检验。结果 在非糖尿病的对照人群中 ,SUR1基因 2 4内含子 - 3t/ c多态性 cc基因型的总胆固醇 (TC)、低密度脂蛋白 (L DL)及载脂蛋白 B(Apo B)水平均比 tt型和 tc型高。家系资料分析结果表明 SUR1基因 2 4内含子 - 3t/ c多态性与 TC、Apo B以及体重指数 (BMI)等表型资料存在显著相关性。结论 在中国北方地区人群中 SUR1基因 2 4内含子 - 3t/ c多态性与 TC、L DL和 Apo

Obeject To study the association of sulfonylurea receptor (SUR) 1 gene and blood lipids level.Method We investigated the SUR1 gene intron 24 3t/c polymorphism by polymerase chain reaction (PCR) and appropriate restriction enzyme (PCR RFLP) in 132 couples of type 2 diabetic case control and 282 type 2 diabetic pedigrees. The statistical methods were t test, multiple line regression and family based association test (FBAT).Result In the control group, the level of total cholesterol (TC), low density lipoprotein (LDL) and Apo B were higher in the cc genotype than that in the tt and the tc genotype. FBAT showed that sulfonylurea receptor 1 gene intron 24 3t/c polymorphism was significantly associated with TC, ApoB and BMI.Conclusion Sulfonylurea receptor 1 gene intron 24 3t/c polymorphism is associated with blood lipid level in north Chinese population.