视黄酸和亚硒酸钠对肝癌细胞某些表型逆转的加和作用

The Additive Effect of Retinoic Acid and Selenite on the Reverse of Some Malignant Phenotypes of Human Hepatocarcinoma Cell Line SMMC-7721

人肝癌细胞株SMMC-7721经1μmol/L视黄酸和或2.5μmol/L亚硒酸钠处理后,膜上纤维连接蛋白沉着量逐日上升,且较相应天数的对照组细胞增加,而甲胎蛋白分泌量和~3H-TdR参入率被明显抑制。视黄酸和亚硒酸钠同时处理的联合组作用强度接近于两者单独使用时作用强度的加和。对以上结果和视黄酸及亚硒酸钠使肝癌细胞接触抑制恢复及表型逆转的关系作了讨论。

After the human hepatocarcinoma cell SMMC-7721 was treated with retinoic acid (1μmoI/L) and/or sodium selenite (2.5μmol/L), it was found that the fibronectin (Fn) on the cell surface was gradually increased during the culture, and Fn content in treated cells was much higher than that of the control. Whereas the secretion of AFP and 3H-TdR incorporation rate into SMMC-7721 cell were suppressed significantly. The effect of the combined use of retinoic acid and sodium selenite was closed to the sum of that of retinoic acid and sodium selenite used separately. The relationship of above mentioned results to the restoration of contact inhibition and the reverse of the phenotype of the hepatocarcinoma cell was discussed.