B细胞慢性淋巴细胞白血病发生Bcl-1基因重排的研究

Bcl-1 rearrangement in B-cell chronic lymphocytic leukemia

目的 探讨Bcl- 1基因重排 (即Bcl- 1/IgH基因重排 )在B细胞慢性淋巴细胞白血病中的发生情况及其临床意义。方法 我们对 13例慢性淋巴细胞白血病 (CLL)进行了Bcl- 1基因重排的检测 ;对照组为 10例骨髓正常者。运用半筑巢式多聚酶链反应 (PCR)技术对从外周血、骨髓中提取的基因组DNA进行扩增。结果 这 13例CLL均为B细胞型 ,在 13例CLL中检出 4例 (31% )的Bcl- 1基因重排 ;10例骨髓正常者的对照组中未检测出Bcl- 1/IgH基因重排。结论 (1)B细胞慢性淋巴细胞白血病常发生Bcl- 1基因重排 ,骨髓正常者未检出Bcl- 1/IgH基因重排。 (2 )发生Bcl- 1基因重排的B细胞慢性淋巴细胞白血病外周血中原始或幼稚淋巴细胞增多。

Objective To study the rearrangement of Bcl 1 gene (Bcl 1/IgH gene rearrangement)in B cell chronic lymphocytic leukemia,and its clinical signification.Methods Hemi nested polymerase chain reaction (PCR) was used to amplify the genomic DNA obtained from fresh peripheral blood and bone marrow.The rearrangment of Bcl 1 gene was analyzed in 13 patients with chronic lymphocytic leukemia (CLL) and 10 volunteers (with normal bone marrow) as controls.Results Four(31%)CLL patients displayed Bcl 1 rearrangem entahile,no volunteers showed the rearrangement of the Bcl 1 gene.All 13 patients with CLL had B cell neoplasms.Conclusions Bcl 1 rearrangement is often present in B cell chronic lymphoproliferative leukemia.B cell chronic lymphoproliferative leukemia with Bcl 1 rearrangement has a higher proportion of lymphoblast and prolymphocytes in peripheral blood.