南鹤虱凝集素的研究

Studies on Lectin from Wild Carrot (Daucus carrot L.) Seed

本文对伞形科16种植物(药用部分)是否存在凝集素进行了筛选。发现野胡萝卜果实与芹菜果实中有凝集素。野胡萝卜果实(中药名称南鹤虱)抽提液的凝血活性较芹菜强。 采用CM-纤维素及DEAE-纤维素对南鹤虱凝集素进行分离纯化。用聚丙烯酰胺凝胶电泳鉴定其纯度。用SDS-聚丙烯酰胺凝胶电泳测定亚基分子量为32600。 此凝集素对热及酸较稳定,对碱略差。对人的A、B、O血型无专一性。能凝集兎、小鼠、牛及鸡的血,但不凝集蟾蜍的血。 此凝集素的凝血活性能被D-木糖及N-乙酰葡萄糖胺轻微地抑制。 经shiff试剂检测表明南鹤虱凝集素为一糖蛋白。糖含量为3.4％。此凝集素分子中的谷氨酸门冬氨酸、精氨酸及絲氨酸的含量较高。

The phytoagglutinin in 16 species from Carrot Families (Umbelliferae) were screened. Lectins were found in the fruit of Daucus carota L. (Wild carrot) and Apium L. (Celery). The agglutinating activity of D. carota L. was stronger than that of Apium L. (Celery).Wild carrot lectin was isolated and purified by chromatography on CM-cellulose and DEAE-cellulose.The purity was identified by polyacrylamide gel electrophoresis. The subunit molecular weight was determined as 32600 by SDS-PAGE.This lectin is stable to heat and acid, but not to alkali. It has no specificity to human blood types. It can agglutinate rabbit, mouse, cattle and chicken erythrocytes, but not toad erythrocyte.The activity of the lectin can be slightly inhibited by D-xylose and N-acetylgl-ucosamine.This lectin was identified to be a kind of glycoprotein which can be stained by shiff's reagent on the polyacrylamide gel. The sugar content is about 3.4% . This lectin contains large amout of glutamic acid, aspartate, arginine and serine.