摘要
降钙素基因相关肽是一个包含有37个氨基酸残基的具有较强降血压生理功能的活性多肽。我们采用常规的固相合成方法,以简单的装置最后经无水氟化氢处理,将肽从树脂上切下,同时脱除所有侧链保护基。粗产物在30％乙酸溶液中用碘作为氧化剂使二个半胱氨酸氧化,形成二硫键。合成的α-人降钙素基因相关肽经反向高效液相层析分离,获得在高效液相层析为单一峰的产物。经酸水解氨基酸分析证明与理论值相符并具有全部生理活性。
The heptatriaconita peptide amide corresponding to the entire amino acid sequence of α-form of human Calcitonin Gene-Related Peptide (α-hCGRP) was synthesized by the solid phase method. All protecting groups employed were removed by treatment with HF and the deprotected peptide was subjected to Iodine to form the intermolecular disulfide bond. After purification by reversed-phase HPLC, a highly purified sample with full biologycal activity of synthetic a-hCGRP was obtained.
关键词
降钙素基因
相关肽
合成
CGRP Solid phase Synthesis
Iodine
Disulfide bond
