摘要
取草莓茎尖,进行离体培养,分化出再生植株、完正植株放于低温(5—10℃)处,壮苗锻炼、获得同步苗、移栽成活率80%以上,通过电镜检测茎尖苗无病毒粒体、无毒草莓所内小区试验获得增产13.9~22.48%的明显效果。
Culture of stem apexes of strawberry was madl in vitro. The regenerated plant were produced. For exr-cising seedlings and obtaining the same stage seedlings, the intact plants were treated in cold temperature (5-10℃). After transfer the seedlings into soli,the survival rate of the seedlings was 80% over. Under electron microscopy, the seedlings from stem aperes were virus-free. The result from plot experiment showed the vims-free strawberries increased 13. 9-22. 48% in yield, compared with virus-infected strawberries.
出处
《生物技术》
CAS
CSCD
1991年第3期30-32,共3页
Biotechnology
关键词
离体培养
再生植株
病毒粒体
草莓
Culture in vitro, plant regeneration, same stage seedling, virus partide