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草莓组织培养研究初报 被引量:2

Preliminary Report on Tissue Culture of strawberry
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摘要 取草莓茎尖,进行离体培养,分化出再生植株、完正植株放于低温(5—10℃)处,壮苗锻炼、获得同步苗、移栽成活率80%以上,通过电镜检测茎尖苗无病毒粒体、无毒草莓所内小区试验获得增产13.9~22.48%的明显效果。 Culture of stem apexes of strawberry was madl in vitro. The regenerated plant were produced. For exr-cising seedlings and obtaining the same stage seedlings, the intact plants were treated in cold temperature (5-10℃). After transfer the seedlings into soli,the survival rate of the seedlings was 80% over. Under electron microscopy, the seedlings from stem aperes were virus-free. The result from plot experiment showed the vims-free strawberries increased 13. 9-22. 48% in yield, compared with virus-infected strawberries.
出处 《生物技术》 CAS CSCD 1991年第3期30-32,共3页 Biotechnology
关键词 离体培养 再生植株 病毒粒体 草莓 Culture in vitro, plant regeneration, same stage seedling, virus partide
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  • 1月德,Yves Desjardins,André Gosselin.草莓组培苗的光合能力与强制通气对其生长的影响[J].园艺学报,1993,20(2):123-126. 被引量:7
  • 2朱文勇,赵玉军,郭黄萍,杜学梅,贺小红.无毒草莓组织培养工厂化快速育苗技术研究[J].山西果树,1995(1):21-22. 被引量:19
  • 3赵望锋,王力华.间歇浸没式生物反应器在大规模组织培养中的应用研究[J].安徽农业科学,2007,35(2):317-320. 被引量:16
  • 4陈振光.园艺植物离体培养学[M].北京:中国农业出版社,1998,89-94.
  • 5覃兰英 徐光霞 等.草莓茎尖离体培养大量繁殖研究初报[J].中国果树,1981,(3):38-41.
  • 6Hempfling T,Preil W.Application of a temporary immersion system in mass propagation of Phalaenopsis. Liquid Culture Systems for in vitro Plant Propagation . 2005
  • 7Berthouly M,Etienne H.Temporary immersion system: a new concept for use liquid medium in mass propagation. Liquid culture systems for in vitro plant propagation . 2005
  • 8Zhao, Yan,Sun, Wei,Wang, Ying,Saxena, Praveen K.,Liu, Chun-Zhao.Improved mass multiplication of Rhodiola crenulata shoots using temporary immersion bioreactor with forced ventilation. Applied Biochemistry and Biotechnology . 2012
  • 9Alister B,Finnie J,Watt M P, et al.Use of the temporary immersion bioreactor system (RITA) for productionof commercial Eucalyptus clones in Mondi Forests (SA). Liquid Culture Systems for in vitro PlantPropagation . 2005
  • 10Ludo Maene,Pierre Debergh.??Liquid medium additions to established tissue cultures to improve elongation and rooting in vivo(J)Plant Cell, Tissue and Organ Culture . 1985 (1)

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