c-kit在血液病中的表达

THE EXPRESSION OF THE PROTO-ONCOGENE(C-KIT) IN MOST KINDS OF HEMATOPATHY

目的 :探讨原癌基因c -kit在血液病中表达的情况。方法 :用Ficoll分离液分离 ,收集骨髓单个核细胞。c -kit的表达采用RT -PCR法。结果 :在不同血液病中c -kit的表达情况不一 ,在急性髓细胞性白血病 (AML)为 5 7.8% ,急性淋巴细胞性白血病 (ALL)为 8.3 % ,骨髓增生异常综合征 (MDS)为 40 %。结论 :c -kit主要在AML中表达 ,提示c -kit表达是粒细胞的一个特异性标志。

Objective:To investigate the expression of the proto-oncogene,c-kit,in 78 cases with most kinds of hematopathy.Methods:Bone marrow mononuclear cells were obtained from leukaemia patients by Ficoll-Conray density-gradient centrifugation.The expression of c-kit was detected by the reverse transcriptase polymerase chain reaction(RT-PCR).Results:c-kit was found about 57.8% in AML(the diagnosis of leukaemia was established by morphology and cytochemistry according to the French-American-British,FAB and immunologic features), c-kit gene shows a high specificity for AML;whereas the expression of c-kit is low in MDS (40%) ,CML (11.1%) ,ALL (8.3%) ,and absent in CLL,AA,MM,ITP,IDA,etc.Conclusions:The expression of c-kit is high in AML.