血管内皮生长因子在卵巢癌细胞浸润转移中的作用

The role of vascular endothelial growth factor on ovarian cancer invasion.

目的 :探讨血管内皮生长因子 (VEGF)在卵巢癌浸润转移中的作用。方法 :阳离子脂质体介导VEGF165质粒转染 2株卵巢癌细胞CaOV 3、COC1,并经逆转录聚合酶链式反应 (RT PCR)、Western免疫印迹检测转染VEGF165质粒前后癌细胞VEGFmRNA及其蛋白表达水平 ;用Boyden小室体外侵袭实验比较VEGF165质粒转染前后以及单克隆抗体作用后 2株癌细胞通过人工重组基底膜的能力强弱变化。结果 :VEGF165质粒转染后 ,2株细胞mRNA和蛋白表达水平均较转染前明显增强 (P <0 .0 5 )。VEGF165质粒转染的癌细胞CaOV 3、COC1穿过人工重组基底膜的相对百分率分别为 4 2 .5± 4 .1、2 6 .8± 2 .4 ,明显高于对照组穿膜细胞相对百分率 (2 4 .7± 1.9、8.6± 1.1) (P <0 .0 5 ) ;而在VEGF单克隆抗体存在时 ,2株癌细胞穿膜细胞相对百分率 (10 .2± 0 .7、5 .4± 0 .3)较处理前均有不同程度的下降 (P <0 .0 5 )。结论 :VEGF参与了卵巢癌转移的侵袭、浸润阶段 ,应用VEGF单克隆抗体可针对性地抑制VEGF介导的卵巢癌侵袭、浸润。

Objective:To explore the role of vascular endothelial growth factor (VEGF)in ovarian tumor metastasis.Methods:The VEGF 165 cDNA was transfected into ovarian tumor cell lines CaOV 3,COC1 by liposome .Transferred cells were screened for VEGF mRNA expression by RT PCR and for VEGF protein expression by Western blot analysis.The in vitro invasion ability of two ovarian tumor cell lines CaOV 3,COC1 was determined with Boyden chamber invasion assay before and after VEGF cDNA transfection and an anti VEGF monoclonal antiboby treatment.Results:The expression of 454bp VEGF mRNA and 36 KD VEGF protein were induced after VEGF 165 cDNA transfection ,which were detected with RT PCR and Western blot.After VEGF 165 cDNA transfected,the mean invasion percentage of two ovarian tumor cells (CaOV 3:42.5±4.1;COC1:26.8±2.4) was higher than that of before (CaOV 3:24.7±1.9;COCl:8.6±1.1)(P<0.05).The in vitro invasive capacity in both two cell lines was reduced after anti VEGF monoclonal antibody treatment (CaOV 3:10.2±0.7;COC1:5.4±0.3)(P<0.05).Conclusions:VEGF correlates tightly with the in vitro invasion of tumor cells,and VEGF may play an important role in invasion in the process of tumor metastasis.