莫桑鼻给非鲫滤泡闭锁中液晶形成的冰冻蚀刻研究

STUDY ON THE FORMATION OF LIQUID CRYSTALS DURING THE FOLLICULAR ATRESIA OF Tilapia mossambica BY FREEZE-ETCHING

本文运用电镜的冰冻蚀刻术研究了莫桑鼻给非鲫滤泡闭锁中液晶形成的过程.结果表明,卵巢内的颗粒细胞吞噬大量的卵黄物质,消化后形成同心圆片层体,这是一种类脂加水以及镶嵌少量的蛋白质的溶致液晶态;细胞内的酶类参与液晶的形成;同时讨论了生物体内相变及液晶态存在的意义.

The formation of liquid crystals during the follicular atresia of Tilapia mossambica was studied by electron microscope with freeze-etching. The results show that the concentric lamolar bodies can be formed after digestion of yolk spheres in granulosa cells The lamellar bodies belong to the lyotropic liquid crystals, which consist of lipids with a few proteins 4- water. It is possible that the enzyme action of the lysosomes in a granulosa cell is at least one of the important factors causing the formation of liquid crystals. The phase transition was also revealed during the digestion of yolk spheres, from gel phase to liquid crystal phase.It is likely that the lipids and some proteins of yolk in a liquid crystal state take part in the metabolism of organism.