亚油酸体系脂质过氧化起动机理的ESR研究

ESR STUDY ON THE INITIATION MECHANISM OF LIPID PEROXIDATION FOR LINOLEIC ACID SYSTEM

本研究直接用含两个双键的不饱和脂肪酸一亚油酸(LH)作为模型化台物,利用自旋捕集ESR技术鉴测脂质过氧化反应的中间自由基L·.研究结果表明:0H自由基,H O,0(?)自由基都不能起动亚油酸的过氧化,即使这种亚油酸含有过氧化成份-LOOH.Fe(Ⅱ)和Fe(Ⅲ)都能使含有过氧化成份的亚油酸产生进一步的过氧化,但不能起动纯的亚油酸的过氧化.

The initiation mechanism of peroxidation of linoleic acid has been studied by ESR spin trapping technique. A water soluble spin trap α-(4-pyridyl-1-oxide) -N-t-butylnitrone (4-POBN) was used to capture an intermediate free radical the carbon-centered radical of linoleic acid (L.) during the reaction of lipid peroxidation. The results show that neither hydroxyl radical ('OH), H2O2 nor superoxide radical (O2) can initiated the peroxidation of linoleic acid within one hour reaction time, even if the linoleic acid contains a little lipid peroxide (LOOK) . Both Fe (Ⅱ) and Fe (Ⅲ) can only continue peroxidation of the linoleic acid with the existence of LOOH, but they can not initiate the peroxidation of pure linoleic acid.