摘要
运用荧光偏振技术了解单纯疱疹病毒(HSV)I型Sm44株感染非洲绿猴肾(Vero)细胞后细胞膜系统脂质分子流动性的变化.结果表明,在所观察的感染后的5个时相中(感染后0、7、14、21、28小时),感染早期(感染后0、7小时)细胞膜的流动性变化不大;感染晚期(14至28小时)细胞膜的脂质流动性明显增强,与未感染病毒的对照组细胞有显著差异,这提示HSV感染晚期的装配,成熟与释放是与被感染细胞的生物膜系统密切相关的.本文对运用该生物物理技术研究病毒和宿主细胞相互关系的意义进行了初步讨论.
Change in membrane fluidity of lipid molecules in herpes simplex virus type I, Strain sm44, infected Vero cells was studied by fluorescence polarization technique with 1, 6-Diphenyl-1, 3, 5 hexatriene as a fluorescent probe. The result showed that in the observed 5 phases (0,7,14,21,28 hours of postinfection) ,there was no dominant difference in membrane fluidity between infected and normal cells in the early phases(0,7 hours of postinfection), but in the late phases(14, 21, 28 hours of postinfection) the membrane fluidity of the infected cells increased evidently. This suggests that there is a close relationship between late events of HSV morphogenesis, such as maturation and release, and the host cell membrane system. The change in membrane fluidity of infected cells was related to the degree of cytopathetic effect of the infected cells and the amount of descendant virus.
出处
《生物物理学报》
CAS
CSCD
北大核心
1991年第4期485-488,共4页
Acta Biophysica Sinica