纳洛酮联用盐酸异丙嗪对偏二甲基肼中毒鼠存活率影响的研究

Effect of combine using Naloxone and Promethazine on mice intoxicated by rocket popellant(UDMH)

目的 本文探讨纳洛酮联用盐酸异丙嗪对火箭推进剂偏二甲基肼 (UDMH)中毒鼠的救治效果 ,以探讨简便易行的对UDMH中毒防治的新途径。方法 LACA雄性小鼠 ,18～ 2 2g ,共 45只。按体重随机分成 5组 :对照组、盐酸异丙嗪组、纳洛酮组、纳洛酮 +盐酸异丙嗪联合用药组、维生素B6组。模型制作 :取 0 2 5LD50 UDMH剂量给各组小鼠腹腔注射。治疗方法 :对照组给予小鼠腹腔注射生理盐水 0 2mL/只 ;纳洛酮组给予小鼠腹腔注射纳洛酮 (0 12mg/kg) 0 2mL/只 ;盐酸异丙嗪组给予小鼠腹腔注射盐酸异丙嗪 (7 5mg/kg) 0 2mL/只 ;联合用药组给予小鼠腹腔注射纳洛酮及盐酸异丙嗪各 0 1mL/只 ;维生素B6组给予小鼠腹腔注射维生素B6(9 0 1mg/kg) 0 2mL/只 ,中毒后 15min、1h分别腹腔内注射上述药物共 2次。注射完毕后观察小鼠的表现和存活情况。结果 5组实验动物中 ,纳洛酮、盐酸异丙嗪组的存活率分别为 33%和 44 % ,高于对照组(11% ) ;以纳洛酮 +盐酸异丙嗪联合用药组的存活率为最高 (5 6 % ) ;但都低于维生素B6组的存活率 (78% )。结论 纳洛酮联用盐酸异丙嗪可明显推迟UDMH中毒小鼠痉挛的发生 ,提高小鼠的存活率。

Objective To investigate effect of combine using Naloxone and Promethazine on mice intoxicated by rocket propellant(UDMH).Methods 45 male LACA mice were divided randomly into 5 groups according to their body weight (18～22 g).The five groups are control group,Promethazine group,Naloxone group,VitB 6 group and combine using of Naloxone and Promethazine group.Each group was given UDMH 0.25 LD50,and then given Nattrichloride(0.9%),Naloxone(0.12 mg/kg),Promethazine(7.5 mg/kg),VitB 6(9.01 mg/kg)and Naloxone(0.12 mg/kg)combined with Promethazine(7.5 mg/kg)respectively.The survival ratio and symptom were observed after injection.Results The finally survival ratio suggested that combine using Naloxone and Promethazine could postponde the mice dying from intoxication with UDMH significantly.Conclusion Combine using Naloxone and Promethazine may play a key point in antagonizing the intoxication of UDMH and could promote the survival ratio