DNA多分子缩合理论模型

A THEORETICAL MODEL OF MULTI-MOLECULAR CONDENSATION OF DNA

在多分子缩合的实验研究基础上,本文以Tanford的递次结合模型为基础,建立了DNA多分子缩合的理论模型.此模型描述了缩合粒子的多分子结合特性,并预测缩合粒子的聚合数分布.模型的理论分布与作者用电镜获得的统计实验分布符合一致.同时,由于一组参量可同时拟合两种不同DNA长度的复曲面分布曲线,因而从理论上阐明缩合粒子的尺度独立于DNA的分子量.此外,还以高斯分布对复曲面的聚合数分布进行了模拟与比较.

With our experimental data of DNA condensation, a theoretical model of multi-molecular condensation of DNA has been built based on the successive association model of TANFORD, It reveals the association mechanism of DNA molecules in a condensed particle, and is able to predict the aggregation number distribution of a condensed particles. The theoretical distribution from model is consistent with the statistical distribution from E.M. data. Since one set of parameters could fit two curves of size distribution of toroids produced fron different length of DNA fragments. it proves that the dimension of condensed particles is independent of the molecular weight of DNA. Besides, the comparison of Gaussian distribution and aggregation number distribution of toroids has been processed.