枳实对大鼠结肠肌条运动的影响

EFFECT OF FRUCTUS AURANTII IMMATURUS ON CONTRACTILE ACTIVITY OF COLONIC MUSCLE STRIPS IN RATS

目的 :观察枳实对大鼠离体结肠肌条收缩活动的影响。方法 :结肠肌条在盛有 5mlKrebs液(37℃ )并供给 95 %O2 和 5 %CO2 混合气体的灌流肌槽中孵育 ,记录肌条的自发收缩活动。分别观察加入枳实、酚妥拉明、普萘洛尔或L NNA对肌条收缩活动的影响。结果 :枳实可显著减小结肠肌条的平均收缩幅度及频率。该作用可被酚妥拉明部分阻断 ,但不受普萘洛尔或L NNA的影响。结论 :枳实可抑制大鼠离体结肠肌条的收缩活动 ,该抑制作用部分与α受体有关。

Objective:Experiments were performed to investigate the effects of Fructus Aurantii Immaturus on contractile activity of isolated colonic muscle strips in rats.Methods:Each strip was suspended in a tissue chamber containing 5ml Krebs solution(37℃)bubbled continuously with 95%O 2 and 5%CO 2.Motility of each strip was simultaneously recorded.The effects of Fructus Aurantii Immaturus,phentolamine,propranolol and L-NNA on the contractile activity of the strips were observed respectively.Results:Fructus Aurantii Immaturus decreased the mean contractile amplitude and frequency of each strip.The effects were blocked partly by phentolamine and were not influenced by propranolol or L-NNA.Conclusion:Fructus Aurantii Immaturus can inhibit the motility of the isolated colonic muscle strip in rats.This inhibitory effects is partly relevant with α receptor.