慢性乙肝病情进展中病毒复制程度的研究

LEVELS OF VIRAL REPLICATION IN PATIENTS WITH CHRONIC HEPATITIS B

目的:研究慢性乙型肝炎病情进展中病毒复制程度的变化并探讨其原因。方法 :采用FQPCR法对慢性乙型肝炎病人进行血清HBVDNA定量检测 ,并与血清ALT和TSB进行相关性分析。结果 :轻、中、重度慢性乙型肝炎HBeAg阳性率分别为 72 .1% ,6 0 .5 %和 4 0 %。HBVDNA阳性率分别为 78.7% ,6 8.4 %和 5 3.3% ,轻、重度两组比较有显著性差异 (P <0 .0 0 5 )。HBVDNA定量在轻、中、重度组分别为 (8.2 6± 1.12 ) ,(7.95±1.0 2 )和 (7.35± 0 .94 ) ,轻、重度两组比较有显著性差异 (P <0 .0 5 )。血清HBVDNA定量与血清ALT和TSB呈负相关 (P <0 .0 1,P <0 .0 2 )。结论 :在慢性乙型肝炎病情进展中 ,HBV复制程度逐渐下降。

Objective:To study the change of HBV replication during clinical progression of the chronic hepatitis B and the cause of the change. Methods:Serum HBVDNA was determined by FQPCR.Correlated bivariate analysis was used between HBVDNA and ALT or TSB.Results:In Mild,medium and severe degrees patients,positive ratio of HBeAg were 72.1%,60.5%,40%,respectively.Positive ratio of HBVDNA were 78.7%,68.4%,53.3%,respectively.The severe degree patients' positive ratio was significantly lower than mild degree patients'( P < 0.005 ).HBVDNA quantities were (8.26±1.12),(7.95±1.02),(7.35±0.94),respectively,in patients with mild,medium and severe degrees.The quantity in severe degree group was significantly lower than mildd dergree group ( P < 0.05 ).Serum HBVDNA quantity was negatively correlated with ALT and TSB ( P <0.01, P <0.02).Conclusions:From mild degree to severe degree,the level of HBV replication decreases gradually.Cytotoxic effect of CTL is an important cause.