选择性毁损帕金森病大鼠模型的建立

ESTABLISHMENT OF SELECTIVITY-LESIONED PD RAT MODEL

目的探讨提高 6 -羟基多巴胺 (6 -OHDA)帕金森病 (PD)大鼠模型成功率的方法 ,并对模型进行评价。方法 :取SD大鼠 90只 ,将 6 -OHDA立体定向微量注射于左侧黑质区及黑质纹状体通路 ,观察大鼠行为及黑质细胞形态学变化。结果 :90只大鼠中经阿朴吗啡诱导后有 6 4只 (占 71.1% )恒定转向右侧且结果稳定 ,旋转圈数 >2 10r/ 30min ,被视为成功PD大鼠模型 ;免疫组化观察发现注射侧黑质区多巴能神经元 (NDN)较对侧明显减少 ,电镜观察发现其普遍存在凋亡及坏死样改变。结论 :用 6 -OHDA选择性损毁NDN可较快建立稳定的类似于人类中晚期PD行为病理的大鼠模型 。

Objective:To determine the methods of increasing success rate of 6-OHDA-lesioned PD rat model and evaluate the model. Method:The changes of behavior and NDN morphology were observed after stereotoxic microinjection of 6-hydroxydopamine(6-OHDA) into the left SNC and VTA in 90 SD rats.Results:①64 of 90 rats showed steadily right apormorphine-reduced rotation (71.1%),and its rotation rate was above 210r/30min,which were regarded as success PD rats.②The immunohistochemical staining showed significant loss of TH-IR neurons in the injection place compared with opposite side.Observation under electron-microscope found that apoptosis and necrosis existed extensively in mid-brain nigra neurons.Conclusions:We can soon estabilish steady PD rat similar to PD of middle or later period in behavior and pathology through 6-OHDA-selectivity-lesioned NDN,but there is some difference in some espects such as behavior and course between the PD rats and PD patients.