聚合酶链反应-微孔板杂交技术检测临床标本中结核分枝杆菌

EVALUATION OF PCR-MICROWELL PLATE HYBRIDIZATION FOR DETECTING MYCOBACTERIUM TUBERCULOSIS IN CLINICAL SPECIMENS

目的评价聚合酶链反应 (PCR) -微孔板杂交技术检测临床标本中结核分枝杆菌的价值。方法 :应用结核病细菌学涂片、培养常规检测方法及PCR -微孔板杂交技术检测 138例结核病患者痰标本 ,4 2例非结核呼吸系统疾病患者痰标本。结果 :用常规细菌学方法检测临床标本中结核杆菌敏感度为 37% (5 1/ 138) ,用PCR-微孔板杂交技术检测敏感度为 5 6 % (77/ 138) ,高于常规检测法 19%。用PCR -微孔板杂交技术检测临床标本特异性为 10 0 %。结论 :PCR -微孔板杂交技术将PCR扩增、核酸杂交的技术及酶免技术相结合 ,简便、快速、敏感度高、特异性强 ,是结核病辅助诊断的有效方法之一。

Objective:To evaluate the value of detecting mycobacterium tuberculosis in clinical specimens by PCR-Microwell Plate hybridization. Method:The sputum specimens of 138 patients with pulmonary tuberculosis and 42 patients with respiratory system diseases other than tuberculosis were tested by smears,cultures and PCR-Microwell Plate hybridization.Results:The sensitivity of detecting mycobacterium tuberculosis in sputum specimens of patients with pulmonary tuberculosis was 37% by smears or/and cultures,and 56% by PCR-Microwell Plate hybridization.The specificity of testing clinical specimens was 100% by PCR-Microwell Plate hybridization. Conclusions:PCR-Microweel Plate hybridization is simple and rapid.This assay,because of its high sensitivity and specificity,is an effective method for assistance diagnosis of tuberculosis.