血管紧张素Ⅱ1型受体基因、血管紧张素Ⅰ转化酶基因多态与2型糖尿病肾病的关系

ASSOCIATION BETWEEN AT1R AND ACE GENE POLYMORPHISM AND CHINESE TYPE 2 DIABETES WITH NEPHROPATHY

目的 :研究血管紧张素Ⅱ 1型受体 (AT1R)A1166C多态及血管紧张素Ⅰ转化酶 (ACE)基因I/D多态与中国汉族 2型糖尿病肾病 (DN)的关系。方法 :运用PCR和PCR/DdeI酶切技术 ,检测 111例DN患者与 138例无DN患者AT1R基因及ACE基因多态基因型。结果 :DN组和无DN组相比 ,AT1R基因A1166C多态基因型频率无差异 (P >0 0 5 ) ;2组间AT1R基因A1166C多态等位基因频率也无差异 (P >0 0 5 ) ;DN组ACE基因DD基因型和D型等位基因频率均升高 (P <0 0 5 ) ;ACE基因DD基因型在AT1R基因AA、AC和CC 3种基因型患者中的分布有差异 (χ2 =7 82 ,P <0 0 5 )。结论 :ACE基因I/D多态性与 2型糖尿病并发DN有关 ,携带DD基因型和D型等位基因的 2型糖尿病患者是DN的易感人群。AT1R基因A1166C多态性不参与 2型糖尿病DN的发病。ACE基因I/D多态和AT1R基因A1166C多态在DN的发生、发展中均为微效基因 ,AA、AC、CC基因型患者携带D型等位基因易发生DN ,ACE。

Objective:To study the association between AT1R,ACE gene polymorphism with diabetic nephropathy(DN) in Chinese type 2 diabetics.Methods:PCR and PCR/Ddel techniques were used to determine the ACE and AT1R gene polymorphism in Chinese type 2 diabetics with nephropathy(n=111) and without nephropathy(n=138).Results:There was no difference of AT1R genotypes between DN and non nephropathy group(P>0.05).There was also no difference of AT1R allele frequencies between DN and non nephropathy group(P>0.05).D type allele and DD genotype distribution were prevalent in DN patients than in non nephropathy patients(P<0.05).The distribution rate of DD genotype in DN group was higher than that in non nephropathy group(χ 2=7.82,P<0.05).Conclusion:It is suggested that AT1R A1166C polymorphism is not associated with DN while ACE gene I/D polymorphism is,they are both microeffective genes and have synergistic effects.It is available to determine ACE gene genotype in DN patients for early prognosis and treatment.