摘要
目的 :分析小儿单纯性肥胖纤溶酶原激活抑制物 1(PAI 1)基因 4G/ 5G多态性及其与血循环PAI 1水平变化的关系 ,探讨儿童期肥胖对纤溶系统活性的影响和有无与之相关的遗传倾向。方法 :用ELISA方法检测 10 5例单纯性肥胖症儿童和 81例正常对照儿童血浆PAI 1含量 ,同时应用聚合酶链反应加限制性片段长度多态 (PCR RFLP)技术检测PAI 1基因 4G/ 5G多态性 ,并分析不同基因型肥胖儿童血循环PAI 1水平的变化。结果 :肥胖儿童血浆PAI 1含量显著升高 (t =5 .0 84 ,P <0 0 1) ,PAI 14G/ 4G、4G/ 5G、5G/ 5G基因型频率无明显变化 (χ2 =1.70 8,P >0 0 5 ) ,4G/ 4G基因型肥胖儿童血浆PAI 1含量显著高于其他两种基因型者 (t分别为 2 117和 2 6 12 ,P均 <0 0 5 )。结论 :肥胖儿童纤溶系统活性降低 ,与PAI 1基因4G/ 5G多态性无明显相关 ,但 4G/ 4G基因型肥胖儿童更易致纤溶活性降低。
Objective:To explore the effect of childhood obesity on fibrinolytic activity and possible associated genetic predisposition by analyzing 4G/5G polymorphism of plasminogen activator inhibitor-1 (PAI-1) gene and its relationship to plasma level of PAI-1.Methods:Plasma concentration of PAI-1 was detected with ELISA and 4G/5G polymorphism of PAI-1 gene with polymerase chain reaction plus restricted fragment length polymorphism (PCR-RFLP) in 105 children with simple obesity and 81 normal-weighted children.Correlation between plasma level of PAI-1 and genotype in children with simple obesity was analyzed.Results:Plasma level of PAI-1 increased significantly( t =5.084, P< 0.001).PAI-1 4G/4G,4G/5G and 5G/5G genotype frequencies had no marked changes ( x 2=1.708, P >0.05).Obese children with 4G/4G genotype had higher circulating level of PAI-1 than the other two genotype ( t =2.117 and 2.612 respectively, P <0.05).Conclusion:There is depresed fibrinolytic activity in children with simple obesity,which isn′t related to 4G/5G polymorphism of PAI-1 gene.Obese children with 4G/4G homozygote manifest reduced fibrinolytic activity relatively easily.
出处
《山东医科大学学报》
2001年第5期391-393,共3页
Acta Academiae Medicinae Shandong
基金
山东省科委科研基金资助课题 ( 9712 2 6 6 0 2 )
