血管细胞粘附分子在胃癌中的表达及其临床意义

Expression of Vascular Cell Adhesion Molecule-1 in Gastric Carcer and It s Clinical Significance

目的 :研究胃癌瘤体中血管细胞粘附分子 ( VCAM- 1)及外周血血清中可溶性血管细胞粘附分子 ( s VCAM- 1)的表达与胃癌的血管生长、转移方面的影响。方法 :对 4 1例胃癌、8例良性胃溃疡、10例正常人标本进行免疫组化染色和图像分析 ,同时用酶联免疫吸附试验对 2 5例正常人、3 0例胃癌术前和术后 1周外周血进行检测 ,分析瘤体及外周血清中 VCAM- 1的表达与胃癌病理参数的关系。结果 :VCAM- 1不仅在胃癌血管内皮细胞上高表达 ,在胃癌细胞膜上也有表达 ,而且 VCAM- 1的表达强度、表达率与胃癌血管的生长和转移有关。胃癌组病人外周血清中 s VCAM- 1的浓度比正常对照组高 ( P<0 .0 1) ,并且术后胃癌病人血清中 s VCAM- 1的浓度下降 ( P<0 .0 1)。结论 :VCAM- 1在胃癌的血管生成、癌细胞转移中可能起到重要作用 ,s VCAM- 1有望成为胃癌的早期诊断及复发的指标。

Objective:To study the correlation of both expression of VCAM 1 (Vascular cell adhesion molecule 1) and gastric cancer circulating sVCAM 1 (soluble vascular cell adhesion molecule 1) with angiogenesis and meta stasis of cancer. Methods: Specimens from 41 patients with gastric cancer, 8 with pept ic ulcer, 10 normal peoples were analysed with the methods of immu nohistochemistry. Meanwhile, the serum levels of soluble VCAM 1 were m easured with enzyme linked immunosorbentassay in 41 patient s with gastric cancer before and after operation. The correlation of VCAM 1 exp ression and pathological characteristics of gastric cancer was studied. Results:VCAM 1 was expressed not only in vascular endothelial cells, but also in gastric cancer cells, serum concentration of VCAM 1 was elevated in the patients with gastric cancer in compared with healthy subje cts ( P<0.001 ), and the levels of postoperative sVCAM 1 was signific antly lower than preoperative ( P<0.001). Conclusion:VCAM 1 ma y play an key role in the angiogenesis, metastasis of gastric cancer, and sVCAM 1 may be considered as an effective diagnostic and prognostic factor.