猕猴实验性内毒素休克早期血浆炎症相关细胞因子水平的改变

Changes of Plasma Cytokine Levels in Early Endotoxic Shock of Monkey Indu ced by Lipopolysaccharide

目的 :研究猕猴实验性内毒素休克过程中 ,前炎症细胞因子与抗炎性细胞因子血浆水平的变化规律。方法 :采用静脉注射细菌内毒素 ( L PS)制成内毒素休克模型 ,用人细胞因子酶联免疫吸附实验 ( EL ISA)试剂盒 ,测定 2 .8mg/ kg内毒素注射后0、80、160 min时血浆中肿瘤坏死因子 - α( TNF- α)、白细胞介素 - 1β( IL- 1β)、白细胞介素 - 10 ( IL- 10 )、白细胞介素 - 12 ( IL- 12 ) p4 0 /p70的改变。结果 :TNF-α、IL - 10在 80 m in时 ,内毒素休克组显著高于对照组 ,但在 160 min时则降至与对照组相当的水平 ,而IL- 10水平降低 ;IL- 1β、IL- 12 p4 0 / p70在 80 min时水平升高不明显 ,而在 160 min时则内毒素休克组水平升高十分明显。结论 :用人细胞因子酶 EL ISA试剂盒检测猕猴血浆中细胞因子是可行的 ;4种细胞因子的时间变化规律与其他动物模型中基本一致 ,IL-10水平低的原因可能是人的试剂盒对猕猴 IL - 10敏感性低所致。

Objective:To evaluate the changes of plasma proinflammatory and anti inflammatory cytokines in monkey endotoxic shock. Methods: LPS(2.8 mg/kg) was intravenously injected to cynomolgus mo nk ey to prepare the endotoxic shock model, Plasma levels of TNF α, IL 1β, IL 10, IL 12 p40/p70 were determined by human ELISA kits at 0, 80 and 160 min afte r LPS challenge.Results:The levels of TNF α and IL 10 increased earlier and reach ed high concentration at 80 min, but the increased level of IL 10 is lower than T NF α. IL 1β and IL 12 p40/p70 lately reached peak concentrations at 160 min .Conclusion:Human cytokine ELISA kits can be used to measure monkey c yt okines. The changes of cytokines in cynomolgus monkey are similar to those of ot her animal models. The lower concentration of IL 10 may be explained by less se nsitivity human IL 10 ELISA kit to monkey IL 10.