Bcl-2和Fas基因在瘢痕成纤维细胞中的表达

EXPERIMENTAL STUDY OF Bcl-2 AND Fas GENE EXPRESSION IN FIBRO BLAST OF SCAR

目的 研究凋亡基因 Fas/ Apo- 1和抑凋亡相关基因 Bcl- 2在瘢痕形成机制中的作用。方法 采用免疫组织化学方法对 10例正常皮肤、10例增生性瘢痕及 10例瘢痕疙瘩成纤维细胞 Fas/ Apo- 1和 Bcl- 2蛋白的表达进行检测。 Fas蛋白稀释为 1∶ 5 0 ;Bcl- 2蛋白稀释为 1∶ 40 ,阴性对照以 PBS代替一抗。随机选择五个高倍视野 ,计算其细胞平均阳性率。结果 Bcl- 2蛋白在正常皮肤、增生性瘢痕及瘢痕疙瘩中阳性率分别为 6 .78%、38.6 %和 83.2 %。瘢痕疙瘩、增生性瘢痕的表达阳性率高于正常皮肤 ,有非常显著性差异 (P<0 .0 1) ,而瘢痕疙瘩的表达阳性率明显高于增生性瘢痕 (P <0 .0 1)。 Fas/ Apo- 1蛋白在正常皮肤、增生性瘢痕及瘢痕疙瘩中阳性率分别为78.4%、 80 .4%和 84.4% ,三组间无显著性差异 (P>0 .0 5 )。结论 病理性瘢痕的形成与 Bcl- 2基因的过度表达有关 ,而对 Fas基因介导的凋亡不敏感或凋亡受阻 ,亦是导致瘢痕过度增生原因之一。

Objective To explore the effect of Fas/Apo-1 and Bcl-2 gene e xpression on mechanism of scar formation. Methods Immunohi stochemical method was applied to defect the expression of Fas and Bcl-2 protein in fibroblasts f r om 10 cases with normal skin, 10 cases with hypertrophic scar and 10 cases with keloid. Results The positive expression rate of Bcl-2 pro tein in keloid was 83.2%, significantly higher than that in hypertrophic scar(38.6%),( P< 0.01), and the positive expression rate in hypertrophic scar and keloid was higher tha n that in normal skin (6.78%), (P< 0.01). But the positive expression rate of Fas/Apo-1 p rotein was 78.4% in normal skin 80.4% in hypertrophic scar, 84.4% in keloid re spectively, which showed no significant difference among them ( P> 0.05). Conclusion Bcl-2 gene but Fas gene may take part in the formation of pathologic sca r.