HPLC法测定健脑丸中阿魏酸含量

Determination of ferulic acid in jiannaowan

目的 :测定健脑丸中阿魏酸含量。方法 :采用 HPL C法 ,色谱柱为 C1 8,流动相为甲醇 -水 -冰醋酸 (38∶ 6 2∶ 0 .1) ,检测波长为 32 3nm。结果 :平均加样回收率为 99.0 7%。结论 :方法简便、快速、灵敏度高 。

OBJECTIVE:To establish a method for the quality control of Jiannaowan.METHODS:HPLC is employed colume:YWG C 18 ( 4.6 mm×250mm,5μm),mobile phase∶methanol∶water∶hydrochloride (38∶62∶0.1),wavelength:232nm,flow rate:1.0ml.min -1 ,column temperature:room temperature.RESULTS:The recovery is 99.07%.CONCLUSION:The method can be used as standard quality control method. It was convenient,sensitive accurate.