一阶导数紫外分光光度法测定瑞巴派特片中瑞巴派特的含量

Quantitative determination of Rebamipide tablets by the first order derivative spectrophotometry

OBJECTIVE: To establish a method for the assay of Rebamipide tablets by the first orderderivative spectrophtometry. METHODS: A phosphate buffer (pH6.8) was selected for solvent. The distance D between the derivative peak at 219± 1nm and valley at 230±1nm of the first order derivative spectrum obtained by scanning from 270 to 200nm was ased as the quantitative basis. The assay was calculated through comparing the peak-valley distance of sample with the standard. RESULTS: There was a good linear relationship between Rebamipide concentration in the range of 1.1～8.5 μg/ml and the peakvalley distance of derivative spectrum with r=0.9999 The average recovery was 99.5% with RSD of 0.3% (n=6). CONCLUSIONS: The method is simple, rapid, accurate and reproducible for the determination of Rebamipide.

OBJECTIVE: To establish a method for the assay of Rebamipide tablets by the first order derivative spectrophtometry. METHODS: A phosphate buffer (pH6.8) was selected for solvent. The distance D between the derivative peak at 219±1nm and valley at 230±1nm of the first order derivative spectrum obtained by scanning from 270 to 200nm was used as the quantitative basis. The assay was calculated through comparing the peak valley distance of sample with the standard. RESULTS: There was a good linear relationship between Rebamipide concentration in the range of 1.1~8.5 μg/ml and the peak valley distance of derivative spectrum with r=0.9999 The average recovery was 99.5% with RSD of 0.3% (n=6). CONCLUSIONS: The method is simple, rapid, accurate and reproducible for the determination of Rebamipide.