环氧司坦的诱变性研究

Mutagenic Study for Epostane

本文应用中国仓鼠肺细胞(CHL)和小鼠骨髓细胞的染色体畸变,研究了环氧司坦的诱变作用。离体CHL细胞培养分代谢活化和非代谢活化二个系列,环氧司坦剂量分别为0μg/ml、20μg/ml、40μg/ml和80μg/ml。小鼠在体试验剂量为0mg/kg、240mg/kg、480mg/kg和960mg/kg,均一次性灌胃给药,并设阳性对照组。结果表明,离体CHL细胞代谢活化与非代谢活化组和小鼠骨髓细胞各剂量组的染色体畸变率小于5%,而阳性对照分别为76%、51%和42%。各处理组与阴性对照组间经统计学分析,无显著性差异,提示了环氧司坦无细胞学诱变作用。

Experlimental study of epostane was carried out in rats on pharmacology in interce-ptive effects,levels of plasma progesterone.This paper reports the mutagenic effect of epo-stane on chromosomal aberration in chinese hamster lung (CHL) cell in vitro and in bonemarrow cell in mice in vivo. The results showed that the chromosomal aberration frequencies of CHL cell induced byepostane were 4%,3%,2% in 24 hrs,with the S9 mix metabolic activation.Without S9mix metabolic activation,the chromosomal aberration frequencies were 4%,4% 2% in24h hrs,and 4%,3%,1% in 48h,at doses of 80 μg/ml,40 μg/ml,20 μg/ml respectively.Therefore, it was only 0～1% in all normal controls;but the positive group presentedhigh frequencies from 42% to 70%,and 2%,0.5% 0.2% at 960 mg/kg,480 mg/kg,240 mg/kg in bone marrow in mice. Since the occurrence of chromosomal aberration induced by epostane at all coses wasless and not statistically significant, the epostane had no mutagenic effect in animal cell invitro and in vivo.