摘要
利用 RAPD引物对我国杂交稻主要的 31个恢复系进行了 DNA多态性分析。从 15 2个随机引物中筛选出 48个引物扩增恢复系的基因组 DNA,选取 9个引物的扩增结果进行分析 ,共获得 78条带 ,其中 6 1条带为多态性标记 ,每个引物平均提供 8.7个标记信息。由 UPMGA方法得到的聚类分析结果表明了 31个恢复系间的亲缘关系 ,聚类结果与它们的系谱关系基本吻合。
The RAPD analysis was conducted on the main 31 restorer lines of hybrid rice in China. Out of 48 random primers, 9 primers were screened, then amplified the 31 genomic DNAs. Seventy-eight RAPD fragments were generated in all, 61 of them were polymorphic markers. Every primer offered 8.7 markers on average. The molecular phylogenetic trees constructed by UPMGA method showed the genetic relationship among 31 restorer lines, which was identical with pedigree analysis.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2002年第1期1-5,共5页
Chinese Journal of Rice Science
关键词
杂交水稻
随机扩增多态性DNA
恢复系
多态性
亲缘关系
hybrid rice
random amplified polymorphic DNA
restorer line
polymorphism
genetic relationship
