苦菜中可溶性多糖提取工艺研究

Extraction Technology of Soluble Polysaccharides From Wild Patrinia Villosa

苦菜具有治疗痢疾的作用。经研究发现 ,苦菜的茎叶及其浸出物还有较强的抗便秘作用。为了确定苦菜浸出物中抗便秘有效成分 ,对苦菜中可溶性多糖的提取工艺进行了研究 ,对料液比、温度、时间分别进行了单因素试验和 L9(33)正交试验 ,并通过极差、方差分析对提取过程中显著影响提取率的因素进行了统计分析。结果显示最佳工艺为料液比 1∶ 2 0、温度 10 0℃、6 h,用改良的苯酚 -硫酸法测定最佳提取工艺时多糖提取率 ,其提取率 (相对于被提取的苦菜质量 )达到 2 .5 8%。

Patrinia Villosa(P.V.) can cure diarrhea, but it can also do good to constipation. Not only the leaves and the stems can relax the constipation, but also the water solution extracted from P.V. has the similar function. In order to know the effective ingredients to prevent and cure constipation extracted in the water solution, the extraction technology of soluble polysaccharides from wild P.V. was studied, and single factor test and orthogonal experiment design methods (L 9(3 3)) were applied to analyze the influence of each factor in solid liquid ratio, temperature and time on the extraction percent of the polysaccharides from the water solution of P.V.. Experimental results indicated that temperature and solid liquid ratio significantly affect the extraction percent of the soluble polysaccharides. The optimum solid liquid ratio is 1∶20, temperature is 100℃ and the extraction time is 6 hours. In this condition, the extraction percent of soluble polysaccharides is 2.58%.