神经生长因子对兔角膜内皮细胞增殖的影响

Effect of nerve growth factor on proliferation of rabbit corneal endothelial cells

目的 探讨神经生长因子（ＮＧＦ）对角膜内皮细胞增殖的影响。方法 在培养兔角膜内皮细胞的培养液中分别添加 ５Ｕ／ｍｌ，５０ Ｕ／ｍｌ和 ５００ Ｕ／ｍｌ的 ＮＧＦ，加药后第３，７天采用四甲基偶氮唑盐（ＭＴＴ）法，在酶标仪上测定５７０ｎｍ波长处的吸光度值来观测细胞增殖情况。结果 与对照组比较，加药后第３，７天，３组的ＮＧＦ对培养的兔角膜内皮细胞增殖均有促进作用（Ｐ＜０．０１），且呈剂量依赖性。其中５０Ｕ／ｍｌ组及５００Ｕ／ｍｌ组作用强于５Ｕ／ｍｌ组（Ｐ＜０．０５），而５０Ｕ／ｍｌ组和５００Ｕ／ｍｌ组比较作用无差异（Ｐ＞０．０５）。结论 外源性ＮＧＦ对培养的兔角膜内皮细胞增殖有明显的促进作用。

Objective To study the effect of nerve growth factor(NGF) on proliferation of corneal endothelial cells. Methods Rabbit corneal endothelial cells in culture were treated with different concentrations of NGF(5 U/ml,50 U/ml and 500 U/ ml), On 3d and 7d after treated with NGF, MTT assay on 570 um was used to examine the proliferation of the cells. Results At the concentrations of NGF from 5 U/ml to 500 U/ml enhanced cellular proliferation effectively compare with control group (P＜ 0. 01),50 U/ml and 500 U/ml of NGF induced higher increase of proliferation compared with that of 5 U/ml NGF(P ＜ 0. 05 ), but the concentrations of NGF at 50 U/ml and 500 U/ml showed similar promotive effect on proliferation of cells(P ＞ 0.05). Conclusion The results suggest that exogenous NGF can stimulate proliferation of rabbit corneal endothelial cells in vitro.