阿司匹林对胶质瘤细胞生长增殖的影响及诱导NOS表达的实验研究

Influence of aspirin on growth and NOS expression in astrocytoma cell line

目的 观察阿司匹林对胶质瘤细胞生长增殖、NOS表达及NO、癌胚抗原 (CEA)含量的影响并探讨其作用机制。方法 应用MTT法测定不同浓度阿司匹林对胶质瘤细胞的生长抑制率 ;免疫组化法检测NOS的表达 ;Griess法测定培养基中NO含量 ;微粒子捕捉免疫法测定CEA。结果 阿司匹林对胶质瘤细胞具有抑制作用 ,诱导iNOS的表达 ,增加NO的生成 ,并呈浓度依赖关系 ;同时具有降低CEA的作用。结论 阿司匹林可抑制胶质瘤细胞的生长增殖。诱导iNOS的表达 ,增加NO含量可能是其部分机制 。

AIM To investigate the effect of aspirin on the proliferation and NOS expression in astrocytoma cell line, and probe into its mechanism. METHOD The effect of aspirin on the growth of astrocytoma cells was evaluated by MTT assay; NOS protein levels were determined by immunocytochemistry, NO and CEA concentration in the medium were determined by Griess assay and lepton catch immunising method respectively. RESULTS Aspirin inhibited the growth of astrocytoma cells, induced the expression of iNOS, increased the concentration of NO in the medium. The effects of these were centratoin dependent. Moreover, aspirin reduced the concentration of CEA in the medium. CONCLUSION Aspirin inhibits the growth of astrocytoma cell line. Up regulated iNOS expression resulting a increase of NO concentration are ascribed to mechanism of antiproliferation activity of aspirin. CEA is a good indicator in monitoring curative effect of astrocytoma.