吡咯并喹啉醌的分离纯化及对铅中毒小鼠的驱铅作用

Purification of pyrroloquinoline quinone and its therapeutic effect on lead poisoned mice

目的 从微生物中分离纯化得到吡咯并喹啉醌 (PQQ) ,研究其对铅中毒小鼠模型的驱铅效果。方法 通过高效液相层析从微生物培养液中纯化得到PQQ ,测定铅中毒小鼠经PQQ治疗后血液、脑和肝中铅含量的变化 ,并与临床常用药物二乙胺四乙酸钠钙 (EDTA)进行比较。结果 服用较高和较低剂量PQQ的铅中毒小鼠的血铅分别从 1.12 7mg/L下降到 0 .5 96mg/L和 0 .992mg/L。肝铅和脑铅也有明显下降 ,肝铅分别从 4 .6 30mg/kg下降到 2 .880mg/kg和 2 .4 4 0mg/kg ;脑铅从 1.790mg/kg下降到 1.2 5 2mg/kg和 0 .94 0mg/kg。用双样本异方差t值检验 ,各治疗组与染铅对照组比较 ,差异均有显著性 (P <0 .0 5 )。结论 PQQ能降低铅中毒小鼠体内的铅含量。

Objective To purify pyrroloquinoline quinone(PQQ) from bacterium ferment and observe the effect of PQQ on lead excretion in mice with lead poisoning. Methods PQQ was separated from the culture fluid of the microrganism by HPLC.Lead contents were determined in the blood,brain and liver of the poisoned mice treated with PQQ or EDTA to evaluate the effect of PQQ. Results The contents of lead in the blood of the poisoned mice decreased from 1.127 mg/L to 0.596 mg/L or 0.992 mg/L respectively after the mice were given PQQ in either higher or lower dosage.Meanwhile, the lead contents in the liver and brain also decreased remarkably.There were marked differences in lead excretion between the treatment groups and the control group( P <0.05). Conclusion PQQ could significantly increase lead excretion.