还原气化-原子吸收光谱法测定尿中汞的方法研究

Study on a determination method of organic,inorganic and total mercury in urine with reduction-gasification atomic absorption spectrometry

目的 研究还原气化 -原子吸收光谱法分别测定尿中总汞、无机汞和有机汞的方法。方法 尿样中汞 (尿中无机汞和经溴酸钾 -溴化钾处理尿中有机汞生成的无机汞 )以硼氢化钠还原生成汞原子蒸气 ,用原子吸收分光光度仪分别测定其总汞、无机汞和有机汞的含量。结果 该法在 0～ 4 8μg/L范围内 ,直线回归相关系数总汞和无机汞分别为 0 .9992、0 .9990 ,检测限总汞为 0 .0 2 8μg/L、无机汞为 0 .0 2 5 μg/L ,精密度测定相对标准偏差总汞为 2 .5 %～ 4 .5 %、无机汞为 1.1%～ 5 .1% ,加标回收率总汞测定为 97.83%～98.5 6 %、无机汞测定为 95 .2 5 %～ 96 .2 5 %。结论 该方法对尿汞测定是一种准确、灵敏、快速、简便的分析方法。

Objective To study the determination method of organic,inorganic and total mercury in urine with reduction gasification atomic absorption spectrometry. Methods The mercury in urine (inorganic mercury in urine and the inorganic mercury transformed from the organic mercury in urine by the treatment of bromate potassium and bromide potassium) was reduced to mercury atom with sodium borohydride,and then was determined by atomic absorption spectrophotometer in total,organic and inorganic form,separately. Results Within the concentration range of 0～48 μg/L,the correlation coefficients for total and inorganic mercury was 0.999 2 and 0.999 0 respectively.The detecting limit was 0.028 μg/L for total mercury and 0.025 μg/L for inorganic mercury.The variation coefficient of accuracy was 2.5%～4.5% for total mercury determination and 1.1%～5.1% for inorganic mercury.The recovery rates were 97.83%～98.56% and 95.25%～96.25% for the total and inorganic mercury detection respectively. Conclusion It was shown that the method was accurate,sensitive,quick and simple to determine urine mercury.