摘要
目的 研究大鼠肝癌变中突变型P53蛋白(mP53)的表达及 p53基因第6外显子点突变。方法 通过DEN诱发的启动模型和肝癌模型,应用免疫组化、MDT-PCR-SSCP和直接测序技术分别研究mP53表达和 p53基因第6外显子点突变。结果 mP53阳性细胞仅见于癌前个别肝细胞增生结节中,8/18例(44.44%)肝细胞癌呈mP53阳性;8例mP53阳性肝癌中仅有1例(12.50%)被检出p53基因第6外显子发生点突变,即第208位密码子第1碱基C—G颠换。结论 在 DEN诱发的启动模型和肝癌模型中 p53基因突变可发生于大鼠肝癌变之癌前及肝癌形成阶段,且第6外显子突变率低。
Objective To study the expression of mutant P53 protein(mP53) and point mutation in exon 6 of p53 gene during hepatocarcino- genesis in rats. Methods By using immunohistochemical fechnique, MDT-PCR-SSCP and direct sequencing the mP53 expression and point muta- tion in exon 6 of p53 gene in both Solt-Farber model and DEN-induced liver cancer model were detected. Results The mP53 was detectable in a few preneoplastic nodules and 8 of 18 rats with hepatocellular carcinomas(HCC) (44.44% ). Only 1 of 8 HCCs mP53 positive reactivity with a C- G transversion was found. The mutation sat on the fist base of 208th codon. Conclusion p53 gene mutation could occur in precancerous stage as well as in HCC with a low frequency in both Solt-Farber model and DEN-induced liver cancer model.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第8期885-888,共4页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目(39270303)
关键词
肝癌变
突变型P53蛋白
多聚酶链反应
动物实验
肝肿瘤
hepatocarcinogenesis
mutant P53 protein
polymerase chain reaction (PCR)
single strand conformation polymorphism(SSCP)
direct sequencing
point mutation